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KMID : 0363020090390020119
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Journal of Korean Academy of Periodontology
2009 Volume.39 No. 2 p.119 ~ p.128
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Investigation of postnatal stem cells from canine dental tissue and bone marrow
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Jhin Min-Ju
Koo Ki-Tae
Kim Tai-Il
Seol Yang-Jo
Lee Yong-Moo
Ku Young
Rhyu In-Chul
Chung Chong-Pyoung
Lee Chul-Woo
Kim Young-Sung
Kim Su-Hwan
Kim Kyoung-Hwa
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Abstract
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Purpose: The aim of this study was to evaluate the stemness of cells from canine dental tissues and bone marrow.
Methods: Canine periodontal ligament stem cells (PDLSC), alveolar bone stem cells (ABSC) and bone marrow stem cells(BMSC) were isolated and cultured. Cell differentiations (osteogenic, adipogenic and chondrogenic) and surface antigens (CD146, STRO-1, CD44, CD90, CD45, CD34) were evaluated in vitro. The cells were transplanted into the subcutaneous space of nude mice to assess capacity for ectopic bone formation at 8 weeks after implantation.
Results: PDLSC, ABSC and BMSC differentiated into osteoblasts, adipocytes and chondrocytes under defined condition. The cells expressed the mesenchymal stem cell markers differently. When transplanted into athymic nude mice, these three kinds of cells with hydroxyapatite /- tricalcium phosphate (HA/TCP) carrier showed ectopic bone formation.
Conclusions: This study demonstrated that canine dental stem cells have stemness like bone marrow stem cells. Transplantation of these cells might be used as a therapeutic approach for dental stem cell-mediated periodontal tissue regeneration.
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KEYWORD
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stem cells, differentiation, periodontal ligament, regeneration
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